Assembly Summary


Taxonomy

Metdbid METDB_00485
Kingdom Eukaryota
Supergroup Hacrobia
Phylum Haptophyta
Class Prymnesiophyceae
Order Phaeocystales
Family Phaeocystaceae
Genus Phaeocystis
Species sp
Strain name RCC908
Strain name synonyms Biosope_226_FL2-3
TaxNCBI 464236
TaxWoRMS
Readset (paired) RCC908 - ERR3497209
Annotation search Link to associated annotation search function

Environmental parameters

Longitude -73.33
Latitude -33.87
Site Pacific Ocean
Site description Chile Sea
Country CHILE
Habitat marine habitat

Legend for assembly and annotation metrics
  • Good
  • Acceptable
  • Bad

Assembly metrics

Percentage of remapping of all readsets on transcriptom 91.3%
Percentage of good mapping 80%
Number of contigs 78,817
Largest contig 4,940
Number of bases 46,923,366
Contigs average lenght 595.3
Number of contigs with orf 34,681
n50 783
GC 0.65984
Transcriptome analysis project
Origin of the datasets RCC
Assembly Type paired
Trinity version trinity-v2.4.0

Annotation metrics

Transdecoder: Number of predicted proteins
BUSCO against eukaryota_odb9
Complete BUSCOs 49.83% (151)
Complete and single-copy BUSCOs 29.04% (88)
Complete and duplicated BUSCOs 20.79% (63)
Fragmented BUSCOs 28.05% (85)
Missing BUSCOs 22.11% (67)
Total BUSCO groups searched 100.00% (303)
Interproscan: Number of proteins with interproscan match
Pfam
Interproscan ID
Gene ontology ID
KEGG ID
PRINTS
Cmsearch
tRNA
tmRNA
SSU_rRNA_eukarya
LSU_rRNA_eukarya
5_8S_rRNA
Others
Diamond against Uniref90_v7-2019


Associated result files


Reference Assembly

Contigs fasta file RCC-METDB_00485-phaeocystis-sp-biosope226fl2-3-paired.fasta

Reads remapping to assembly

Quantification file (sf) quant.sf
Metadata info (json) RCC_phaeocystis-sp-biosope-226-fl2-3_salmon-v0.9.1_roscoff.json

Assembly metrics

Contigs metrics (csv) contigs.csv
Contigs with good mapping (fasta) good.METDB-00485_phaeocystis-sp-biosope226fl2-3.fasta.gz
Contigs with bad mapping (fasta) bad.METDB-00485_phaeocystis-sp-biosope226fl2-3.fasta.gz
Assembly metrics file (csv) RCC_phaeocystis-sp-biosope-226-fl2-3_transrate-v1.0.2_roscoff.csv

Annotation files

InterProscan: Protein sequence analysis and classification RCC-METDB_00485-phaeocystis-sp-biosope226fl2-3-paired_full_i5_annotations
Diamond: for aligning Transcripts against NCBI NR RCC-METDB_00485-phaeocystis-sp-biosope226fl2-3-paired.diamond_matches
Transdecoder: Find Coding Regions Within Transcripts RCC-METDB_00485-phaeocystis-sp-biosope226fl2-3-paired_cut.cleaned.fasta.transdecoder.pep
BUSCO: assessment of transcriptome completeness RCC-METDB_00485-phaeocystis-sp-biosope226fl2-3-paired_BUSCO_short_summary.txt
Cmsearch: Use of Covariance model (CM) to search for homologous RNAs Within Transcriptoms RCC-METDB_00485-phaeocystis-sp-biosope226fl2-3-paired_cat_cmsearch_matches.tbl.deoverlapped

Quality file

Read quality file BBD_AAEAOSW_2_1_C1JHLACXX.IND7_noribo_clean.fastq.P.qtrim_fastqc.zip
Read quality file BBD_AAEAOSW_2_2_C1JHLACXX.IND7_noribo_clean.fastq.P.qtrim_fastqc.zip